Isolation and Characterization of Two Safflower Oleoyl-Acyl Carrier Protein Thioesterase cDNA Clones
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چکیده
منابع مشابه
Isolation and characterization of two safflower oleoyl-acyl carrier protein thioesterase cDNA clones.
Oleoyl-acyl carrier protein (18:1-ACP) thioesterase has been partially purified from developing safflower (Carthamus tinctorius) seeds. Protein species with molecular masses of 34 and 40 kD associated with thioesterase activity were identified and partially sequenced. Analysis of amino-terminal and internal cyanogen bromide peptide sequences revealed no differences in the primary structure of t...
متن کاملcloning and functional characterization of a fatty acyl-acyl carrier protein thioesterase gene (bnfatb) in brassica napus l.
plant fatty acyl-acyl carrier protein thioesterase (fat) is a major enzyme regulating the amount and composition of fatty acids in lipids. in this study, one type of cdna, corresponding to the fatty acyl-acyl carrier protein (acyl-acp) thioesterase (fat) enzyme, was isolated from the seed of brassica napus cv. ningyou12. blast results revealed that the cdna identified highly with the fatb class...
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We have isolated two cDNA clones (CDES01) and 04) encoding stearoyl-acyl carrier protein desaturase (SACPD; EC 1.14.99.6) from immature sesame seeds, and have analyzed accumulation levels of the corresponding mRNAs at different stages and organs in sesame. Clone CDES01 contains an open reading frame coding for a 396-amino acid protein of 45 kDa. CDES04 encodes a partial sequence of 141-amino ac...
متن کاملPalmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases.
Acyl-acyl carrier protein (ACP) thioesterases play an essential role in chain termination during de novo fatty acid synthesis and in the channeling of carbon flux between the two lipid biosynthesis pathways in plants. We have discovered that there are two distinct but related thioesterase gene classes in higher plants, termed FatA and FatB, whose evolutionary divergence appears to be ancient. F...
متن کاملPalmitoyl-Acyl Carrier Protein (ACP) Thioesterase and the Evolutidnary-Origin of Plant ACyl-ACP Thioesterases
Acyl-acyl carrier protein (ACP) thioesterases play an essential role in chain termination during de novo fatty acid synthesis and in the channeling of carbon flux between the two lipid biosynthesis pathways in plants. We have discovered that there are two distinct but related thioesterase gene classes in higher plants, termed FatA and Fai6, whose evolutionary divergence appears to be ancient. F...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1992
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.100.4.1751